Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves.
Identifieur interne : 003518 ( Main/Exploration ); précédent : 003517; suivant : 003519Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves.
Auteurs : Bahtijor Rasulov [Estonie] ; Lucian Copolovici ; Agu Laisk ; Ulo NiinemetsSource :
- Plant physiology [ 0032-0889 ] ; 2009.
Descripteurs français
- KwdFr :
- Alkyl et aryl transferases (métabolisme), Butadiènes (analyse), Cinétique (MeSH), Composés organiques du phosphore (métabolisme), Environnement (MeSH), Feuilles de plante (effets des radiations), Feuilles de plante (enzymologie), Hémiterpènes (analyse), Hémiterpènes (métabolisme), Lumière (MeSH), Obscurité (MeSH), Pentanes (analyse), Populus (effets des radiations), Populus (enzymologie), Température (MeSH).
- MESH :
- analyse : Butadiènes, Hémiterpènes, Pentanes.
- effets des radiations : Feuilles de plante, Populus.
- enzymologie : Feuilles de plante, Populus.
- métabolisme : Alkyl et aryl transferases, Composés organiques du phosphore, Hémiterpènes.
- Cinétique, Environnement, Lumière, Obscurité, Température.
English descriptors
- KwdEn :
- Alkyl and Aryl Transferases (metabolism), Butadienes (analysis), Darkness (MeSH), Environment (MeSH), Hemiterpenes (analysis), Hemiterpenes (metabolism), Kinetics (MeSH), Light (MeSH), Organophosphorus Compounds (metabolism), Pentanes (analysis), Plant Leaves (enzymology), Plant Leaves (radiation effects), Populus (enzymology), Populus (radiation effects), Temperature (MeSH).
- MESH :
- chemical , analysis : Butadienes, Hemiterpenes, Pentanes.
- chemical , metabolism : Alkyl and Aryl Transferases, Hemiterpenes, Organophosphorus Compounds.
- enzymology : Plant Leaves, Populus.
- radiation effects : Plant Leaves, Populus.
- Darkness, Environment, Kinetics, Light, Temperature.
Abstract
The control of foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyldiphosphate (DMADP), and the pool size of DMADP. Due to limited in vivo information of isoprene synthase kinetic characteristics and DMADP pool sizes, the relative importance of these controls is under debate. In this study, the phenomenon of postillumination isoprene release was employed to develop an in vivo method for estimation of the DMADP pool size and to determine isoprene synthase kinetic characteristics in hybrid aspen (Populus tremula x Populus tremuloides) leaves. The method is based on observations that after switching off the light, isoprene emission continues for 250 to 300 s and that the integral of the postillumination isoprene emission is strongly correlated with the isoprene emission rate before leaf darkening, thus quantitatively estimating the DMADP pool size associated with leaf isoprene emission. In vitro estimates demonstrated that overall leaf DMADP pool was very large, almost an order of magnitude larger than the in vivo pool. Yet, the difference between total DMADP pools in light and in darkness (light-dependent DMADP pool) was tightly correlated with the in vivo estimates of the DMADP pool size that is responsible for isoprene emission. Variation in in vivo DMADP pool size was obtained by varying light intensity and atmospheric CO(2) and O(2) concentrations. From these experiments, the in vivo kinetic constants of isoprene synthase were determined. In vivo isoprene synthase kinetic characteristics suggested that isoprene synthase mainly operates under substrate limitation and that short-term light, CO(2), and O(2) dependencies of isoprene emission result from variation in DMADP pool size rather than from modifications in isoprene synthase activity.
DOI: 10.1104/pp.108.133512
PubMed: 19129417
PubMed Central: PMC2649399
Affiliations:
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Bahtijor" uniqKey="Rasulov B" first="Bahtijor" last="Rasulov">Bahtijor Rasulov</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Physiology, University of Tartu, 51010 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Department of Plant Physiology, University of Tartu, 51010 Tartu</wicri:regionArea><wicri:noRegion>51010 Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Copolovici, Lucian" sort="Copolovici, Lucian" uniqKey="Copolovici L" first="Lucian" last="Copolovici">Lucian Copolovici</name></author><author><name sortKey="Laisk, Agu" sort="Laisk, Agu" uniqKey="Laisk A" first="Agu" last="Laisk">Agu Laisk</name></author><author><name sortKey="Niinemets, Ulo" sort="Niinemets, Ulo" uniqKey="Niinemets U" first="Ulo" last="Niinemets">Ulo Niinemets</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (metabolism)</term><term>Butadienes (analysis)</term><term>Darkness (MeSH)</term><term>Environment (MeSH)</term><term>Hemiterpenes (analysis)</term><term>Hemiterpenes (metabolism)</term><term>Kinetics (MeSH)</term><term>Light (MeSH)</term><term>Organophosphorus Compounds (metabolism)</term><term>Pentanes (analysis)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (radiation effects)</term><term>Populus (enzymology)</term><term>Populus (radiation effects)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkyl et aryl transferases (métabolisme)</term><term>Butadiènes (analyse)</term><term>Cinétique (MeSH)</term><term>Composés organiques du phosphore (métabolisme)</term><term>Environnement (MeSH)</term><term>Feuilles de plante (effets des radiations)</term><term>Feuilles de plante (enzymologie)</term><term>Hémiterpènes (analyse)</term><term>Hémiterpènes (métabolisme)</term><term>Lumière (MeSH)</term><term>Obscurité (MeSH)</term><term>Pentanes (analyse)</term><term>Populus (effets des radiations)</term><term>Populus (enzymologie)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Butadienes</term><term>Hemiterpenes</term><term>Pentanes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Hemiterpenes</term><term>Organophosphorus Compounds</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Butadiènes</term><term>Hémiterpènes</term><term>Pentanes</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Composés organiques du phosphore</term><term>Hémiterpènes</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Darkness</term><term>Environment</term><term>Kinetics</term><term>Light</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Environnement</term><term>Lumière</term><term>Obscurité</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The control of foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyldiphosphate (DMADP), and the pool size of DMADP. Due to limited in vivo information of isoprene synthase kinetic characteristics and DMADP pool sizes, the relative importance of these controls is under debate. In this study, the phenomenon of postillumination isoprene release was employed to develop an in vivo method for estimation of the DMADP pool size and to determine isoprene synthase kinetic characteristics in hybrid aspen (Populus tremula x Populus tremuloides) leaves. The method is based on observations that after switching off the light, isoprene emission continues for 250 to 300 s and that the integral of the postillumination isoprene emission is strongly correlated with the isoprene emission rate before leaf darkening, thus quantitatively estimating the DMADP pool size associated with leaf isoprene emission. In vitro estimates demonstrated that overall leaf DMADP pool was very large, almost an order of magnitude larger than the in vivo pool. Yet, the difference between total DMADP pools in light and in darkness (light-dependent DMADP pool) was tightly correlated with the in vivo estimates of the DMADP pool size that is responsible for isoprene emission. Variation in in vivo DMADP pool size was obtained by varying light intensity and atmospheric CO(2) and O(2) concentrations. From these experiments, the in vivo kinetic constants of isoprene synthase were determined. In vivo isoprene synthase kinetic characteristics suggested that isoprene synthase mainly operates under substrate limitation and that short-term light, CO(2), and O(2) dependencies of isoprene emission result from variation in DMADP pool size rather than from modifications in isoprene synthase activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19129417</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>149</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves.</ArticleTitle><Pagination><MedlinePgn>1609-18</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.108.133512</ELocationID><Abstract><AbstractText>The control of foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyldiphosphate (DMADP), and the pool size of DMADP. Due to limited in vivo information of isoprene synthase kinetic characteristics and DMADP pool sizes, the relative importance of these controls is under debate. In this study, the phenomenon of postillumination isoprene release was employed to develop an in vivo method for estimation of the DMADP pool size and to determine isoprene synthase kinetic characteristics in hybrid aspen (Populus tremula x Populus tremuloides) leaves. The method is based on observations that after switching off the light, isoprene emission continues for 250 to 300 s and that the integral of the postillumination isoprene emission is strongly correlated with the isoprene emission rate before leaf darkening, thus quantitatively estimating the DMADP pool size associated with leaf isoprene emission. In vitro estimates demonstrated that overall leaf DMADP pool was very large, almost an order of magnitude larger than the in vivo pool. Yet, the difference between total DMADP pools in light and in darkness (light-dependent DMADP pool) was tightly correlated with the in vivo estimates of the DMADP pool size that is responsible for isoprene emission. Variation in in vivo DMADP pool size was obtained by varying light intensity and atmospheric CO(2) and O(2) concentrations. From these experiments, the in vivo kinetic constants of isoprene synthase were determined. In vivo isoprene synthase kinetic characteristics suggested that isoprene synthase mainly operates under substrate limitation and that short-term light, CO(2), and O(2) dependencies of isoprene emission result from variation in DMADP pool size rather than from modifications in isoprene synthase activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rasulov</LastName><ForeName>Bahtijor</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant Physiology, University of Tartu, 51010 Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Copolovici</LastName><ForeName>Lucian</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Laisk</LastName><ForeName>Agu</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Niinemets</LastName><ForeName>Ulo</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>01</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009943">Organophosphorus Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance 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